Detection of Chlamydia trachomatis Endocervical Infections by Ligase Chain Reaction versus ACCESS Chlamydia Antigen Assay
نویسندگان
چکیده
منابع مشابه
Detection of Chlamydia trachomatis endocervical infections by ligase chain reaction versus ACCESS Chlamydia antigen assay.
Ligase chain reaction (LCR) was compared with ACCESS immunoassay for detection of chlamydial infections in females. Despite efforts to improve ACCESS performance by evaluation of specimens that were in the test performance "grey zone," LCR remained more sensitive and was less expensive to perform. ACCESS had a sensitivity of 83.9%, a specificity of 99.7%, a positive predictive value of 96.3%, a...
متن کاملA comparison of ligase chain reaction to polymerase chain reaction in the detection of Chlamydia trachomatis endocervical infections.
OBJECTIVE To compare the reliability of ligase chain reaction (LCR) to polymerase chain reaction (PCR) in detecting Chlamydia trachomatis endocervical infections. METHODS We conducted a prospective study of 486 patients at risk for chlamydial infection of the endocervix. We obtained two endocervical specimens from each patient and used LCR and PCR to detect C. trachomatis. Discrepant results ...
متن کاملEffect of endocervical specimen adequacy on ligase chain reaction detection of Chlamydia trachomatis.
Several studies have demonstrated that the sensitivity of a commercially available PCR test for the detection of Chlamydia trachomatis (Roche Diagnostics) is affected by the cellular quality of the endocervical swab specimens. The cellular adequacies of 1,633 female endocervical swab specimens were assessed and compared with the results of C. trachomatis detection obtained by ligase chain react...
متن کاملReproducibility of the Roche Amplicor polymerase chain reaction assay for detection of infection by Chlamydia trachomatis in endocervical specimens.
tion of traditional, iron-laden beer, and two-fifths of them had increased serum ferritin. In contrast to our studies, Wapnick et al. (7) reported that serum iron concentrations increased dramatically after oral administration of vitamin C to iron-loaded, scorbutic subjects. The explanations for this difference probably lie in the timing of the vitamin C dose in relation to the serum iron measu...
متن کاملClinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens.
A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs (CP24-CP27), uracil-N-glycosylase (AmpErase), and a microtiter format for ampl...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1999
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.37.9.3072-3073.1999